JPAC Joint United Kingdom (UK) Blood Transfusion and Tissue Transplantation Services Professional Advisory Committee
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Appendix 3 - Calculation of Blood and Plasma Dilution

Haemodilution algorithm

Appendix 3 – Calculation of Blood & Plasma Dilution

Please find attached an algorithm for determining the suitability of post-transfusion/infusion samples for mandatory screening for transmissible infections.

Determining the Suitability of Post-transfusion/Infusion Samples for Mandatory Screening for Transmissible Infections

Determing sample suitability - Step 1

Determining sample suitability - Step 2

Donor ID:                                        Date & Time blood sample taken:                                                  

STEP 2A

Calculate plasma volume Donor weight (kg) _________
                                      0.025

____________mL
Calculate blood volume Donor weight (kg) _________
                                      0.015		 ____________mL
A) Record total volume of blood transfused in the 48 h prior to death or sample collection (whichever comes first)

______mL of RBC transfused/48 h

______mL of whole blood transfused/48 h

______mL of reconstituted blood/48 h

 Sum A: _______mL
B) Record total volume of colloid infused in the 48 h prior to death or sample collection (whichever comes first)

______mL plasma/48 h

______mL platelets/48 h

______mL albumin/48 h

______mL HES or other colloids/48 h    

 

 Sum B: _______mL
C) Record total volume of crystalloid infused in the 1 h prior to death or sample collection (whichever comes first) ______mL Sum C: _______mL

 

Step 2B

Calculated Plasma Volume ______mL Sum B + Sum C ______mL
Calculated Blood Volume ______mL Sum A + Sum B + Sum C ______mL
Calculate plasma dilution Is Sum B + Sum C > plasma volume? No Yes
Calculate blood dilution Is Sum A + Sum B + Sum C > blood volume? No Yes

If the answers to both questions are ‘No’, the post-transfusion/infusion sample is acceptable

If the answer to either of the questions is ‘Yes’ use a pre-transfusion/infusion sample. If a suitable sample is not available, seek expert advice and inform transplant centre, testing laboratory, tissue bank as necessary.

RBC = red blood cells; HES = hydroxyethyl starch

Based on the algorithm developed by the Food and Drug Administration, USA

www.fda.gov/downloads/BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformation/Guidances/Tissue/ucm091345.pdf (Appendices 2&3)


This appendix was last updated in TDSG-DD Edition 203, Release 32, Issue 01

Last updated 26/07/2018